Awesome! Great work Nate.https://twitter.com/NathanGrubaugh/status/1055783796207992832 …
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Challenge accepted! Positions that look weird to me are 6204 and 10393. They mutate about 50 times on the tree in very similar patterns. https://nextstrain.org/WNV/NA?c=gt-nuc_6204 … Do you wobble primers?pic.twitter.com/nuj6wNR73b
Lots of methods used to generate these from the different labs, but no, we don’t use wobble primers. Primers here: https://docs.google.com/spreadsheets/d/1zMfUv1IV5-Sy-AeOPhKHdoZMUxhD1R_RsB5gPswqx40/htmlview …
One thing that does intrigue (and bother) me is that NS4A-A85T that differentiates WN02 from SW03 pops up multiple times in the tree. Perhaps it’s real, because this isn’t coming from just one lab, but not sure what to make of it. Maybe convergent evo?pic.twitter.com/5FH4kIWbCn
Parallel change is not necessarily adaptive, unless it matches phenotypic/environment changes. Parallel change can also indicate neutral variation.
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