We just did a big push on our West Nile genomics studies and now have >500 genomes from California. Check out the project (and download the data) here:https://andersen-lab.com/secrets/data/west-nile-genomics/ …
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Als antwoord op @K_G_Andersen
Thanks for sharing! Did you characterise defective/DI-RNAs? Think there were some large deletions of the structural genes reported before in WNV.
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Als antwoord op @AndrewRouth @K_G_Andersen
Howdy are these all being passaged in Vero before sequencing? I cant tell if they are are amplicon or Random hexamers.
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Als antwoord op @RhysHParry @AndrewRouth
Sorry, will update with methods shortly - oversight. In summary, no passage before sequencing, but all of these are based on amplicon sequencing using our standard protocol:https://docs.google.com/document/d/1PilT4w5jHO-ROsE8TL5WBGa0wSCdTHAsNl1LIOYiTgk/edit …
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Als antwoord op @K_G_Andersen @AndrewRouth
Goddamn this protocol is clean. Thanks.
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Als antwoord op @RhysHParry @AndrewRouth
Yup -
@NathanGrubaugh doesn’t do anything half-arsed
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And of course - based on all the hard work from @Scalene and @pathogenomenick and many others.
Het laden lijkt wat langer te duren.
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