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  1. Prikvačeni tweet
    23. ruj 2019.
    Odgovor korisnicima

    Happy to confirm that this is NOT fake news! Very excited to start my own lab in Oct 2020. Will work on comparative connectomics and barcodes in the cerebellum and beyond! Would never have happended without my mentors and Liqun Luo.

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  2. 25. sij

    Intriguingly, the connection to lateral habenula is potentiated both when tone and footshock are paired or unpaired.

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  3. 25. sij

    Similarly, SYNPLA works on ex vivo slices of defense conditioned animals. When expressing myc-NRXN in auditory cortex and/or medial geniculate nucleus, we show that paired tone and footshock potentiate the connection to the lateral amygdala relative to controls.

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  4. 25. sij

    In the paper, we show that SYNPLA works in cultured neurons and hippocampal slice cultures. There we detect a much larger number of SYNPLA signals after chemically induced LTP than in control conditions or when LTP is blocked.

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  5. 25. sij

    In SYNPLA we probe for endogenous GluA1 and the pathway-specifically expressed pre-synaptic myc-NRXN. We only get a SYNPLA signal if a synapse contains both GluA1 and myc-NRXN . Extra-synaptic GluA1 is too far away from the presynaptic partner to make a signal!

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  6. 25. sij

    We do this by PLA. PLA is essentially a double antibody stain that results in a 1000x amplified signal only if the two proteins probed for are closer than 40nm to each other. This happens across the synaptic cleft.

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  7. 25. sij

    Synaptic GluA1, therefore, is a marker for recently potentiated synapses. The trick is to detect this synaptic GluA1 only in genetically or anatomically specified pathways.

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  8. 25. sij

    So, what is this paper about? SYNPLA is a method to label synapses in a specific pathway that recently underwent LTP. The principle is simple. We exploit the fact that after LTP GluA1 temporarily moves into the synapse from extra-synaptic pools.

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  9. 24. sij

    New paper out in PNAS today. Long-running collaboration between Malinow and labs brought to fruition by Kim Dore! Check it out. SYNPLA, a method to identify synapses displaying plasticity after learning

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  10. proslijedio/la je Tweet
    16. sij

    Cell-type- and temporally-resolved cell-surface proteomics in intact brains ⁦⁦⁦⁩. Collab. of Liqun's lab, ⁦⁩'s lab, Steve's team ⁦⁩. Thank you all! ⁦⁦⁦⁦

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  11. proslijedio/la je Tweet
    23. pro 2019.
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  12. proslijedio/la je Tweet
    4. pro 2019.

    Thought that frontal cortex is all random mixed selectivity, messy? Think again! Great work by a great team,

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  13. proslijedio/la je Tweet

    “Neocortex–Cerebellum Circuits for Cognitive Processing” Review by Mark Wagner & Liqun Luo,

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  14. proslijedio/la je Tweet
    28. stu 2019.

    Neuroscience opportunities in Jing Ren group: Developmental assembly of the serotonin system 🧠😀 Apply NOW Deadline 3 Dec 2019:

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  15. proslijedio/la je Tweet
    5. stu 2019.

    We had been rather proud of our 20 genome assembly project in Heliconius, and now we find that Nick Grishin's group has sequenced ALL 845 NORTH AMERICAN BUTTERFLY SPECIES! Mental!

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  16. proslijedio/la je Tweet
    25. lis 2019.

    Excited to present a method for profiling cell-surface proteomes in intact tissues, with cell-type, spatial, and temporal specificities. A collaboration of the Luo lab, lab, and the proteomics team . Thank you all!

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  17. proslijedio/la je Tweet
    25. lis 2019.

    Proximity labeling in pupae & adult fly brain reveals changes in neuronal surface proteome over development. New synaptic wiring candidates IDed and 20 validated by fly KD. Great collab w/Liqun Luo & Carr lab. Congrats co-1st authors & Shuo Han!

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  18. 21. lis 2019.

    Are you mapping brain circuits by clearing and light sheet imaging, but have a hard time truely quantifying axonal projections? Check out Drew Friedmann's TrailMap (Luo Lab) now on biorxiv. Then you too can make awesome movies like this one!

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  19. proslijedio/la je Tweet
    17. lis 2019.

    BARseq paper finally out! Connectivity of thousands of neurons per brain, with gene expression, at single neuron resolution, using in situ sequencing.

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  20. 17. lis 2019.

    Liked this whole sequencing projections idea, but want more spatial resolution? BARseq is now published! De novo in situ sequencing of barcodes + single neuron tracing for thousands of cells at a time, now out from the Zador lab. Read it here:

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  21. proslijedio/la je Tweet
    5. lis 2019.

    Looking for students and postdocs to join my lab , starting early 2020! Working at the intersection of molecular and systems neuroscience, we’ll study how cell types in visual cortex are wired and contribute to visual perception. Please RT / get in touch if interested!

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