Juan Carvajal García

@Jucarvag

Doing Genetics and Molecular Biology (UNC Chapel Hill). Biotecnólogo (Universidad de Oviedo). Oviedista. Asturiano en Carolina del Norte.

Chapel Hill, NC
Vrijeme pridruživanja: kolovoz 2011.

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  1. Prikvačeni tweet
    20. pro 2019.

    I present to you the most fun, yet most difficult, thing I have ever done. Here is my first-author manuscript on how DNA Polymerase Theta (POLQ) finds microhomologies to repair chromosome breaks during Theta Mediated End Joining (TMEJ).

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  2. proslijedio/la je Tweet
    prije 15 sati

    ¿Es que no se sabe que en este pueblo es verdadera devoción la que hay por Cuerda? Descansa en paz, genio.

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    Vamos a recordar que el Banco de España dijo que con el SMI se destruirían 125.000 empleos.

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  5. proslijedio/la je Tweet
    17. sij
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  6. 15. sij

    O lo que es lo mismo, el 0.2% de los trabajadores. 1.3 millones de trabajadores fueron los que pasaron a cobrar el sueldo minimo (30 veces mas de los despedidos).

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  7. 15. sij

    The time has come. I think for the first time of my life I've messed up an experiment because of my colorblindness.

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    11. sij
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    Today I'm proud to endorse for president. Elizabeth and I share a vision of America where everyone counts. An America where people⁠—not the wealthy or well-connected⁠—are put first. I'm proud to join her in the fight for big, structural change.

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  10. proslijedio/la je Tweet
    3. sij

    ”Imagine that DNA had a diameter of 1 m. Then the complex that copies the DNA would be the size of a truck. It would be traveling at a speed of 500 km/h. It would be making a delivery on both sides of the street every ~10 cm. It would finish its journey in 40 min... 1/2

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  12. 31. pro 2019.

    No por sabido es menos importante decirlo, es el mejor programa del año.

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    Our house is on fire.

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  14. proslijedio/la je Tweet
    26. pro 2019.

    Templated insertions (TINS) as a hallmarck for the DNA polymerase Theta mediated end joining pathway (TMEJ). by . We've noticed TINS also in Arabidopsis CRISPR mutants!

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  15. proslijedio/la je Tweet
    22. pro 2019.

    Hoy en la ciudad deportiva al finalizar el encuentro contra el "B" nuestro jugador ha tenido que escuchar insultos racistas... Desde nuestro club pedimos a todas las aficiones respeto por todos los jugadores, sean del país, nacionalidad o raza que sean!

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  16. proslijedio/la je Tweet
    20. pro 2019.

    Take-home message from Juan: Stop calling it MMEJ!

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  17. 20. pro 2019.

    Last, but not, least, we highlight how MMEJ probably encompasses three different genetic pathways (NHEJ, TMEJ and a yet uncharacteristic one that is suppressed by KU and POLQ). Here are some people that helped me with this:

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  18. 20. pro 2019.

    However, templated insertions are way more specific! We can correlate them with high POLQ expression and mutations in BRCA1/2 in the genomes of breast tumors!

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  19. 20. pro 2019.

    …or by extending from a harping. This creates templated insertions, a very specific signature of POLQ/TMEJ activity. Microhomology mediated deletions have been proposed as a way to identify high TMEJ activity in tumor genomes, but other end joining pathways also use them!

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  20. 20. pro 2019.

    This is a great way to minimize the loss of DNA associated with TMEJ! But what happens when there are no “good” microhomologies within whose 10-15 nucleotides? POLQ makes new ones! Either by extending from “bad” microhomologies, failing, and re-annealing at newly made ones…

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  21. 20. pro 2019.

    We figured out that POLQ starts searching for microhomologies at the 3’ ends of ssDNA tails and moves inwards, most often using the one most proximal to the end. Also, there is a limit to how far it searches, between 10 and 15 nucleotides.

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