The E. coli relationship was steeper for G->A mistranscription events. It was non-existent for C->U because too much deamination happened during sample prep rather than biologically.pic.twitter.com/MPS5RwK3Fp
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The E. coli relationship was steeper for G->A mistranscription events. It was non-existent for C->U because too much deamination happened during sample prep rather than biologically.pic.twitter.com/MPS5RwK3Fp
S. cerevisiae has a much lower error rate, and no relationship - except for the C->U errors, which were measured with a better protocol, and are at high enough rates for selection to care.pic.twitter.com/xI4B6V0FTq
Curves like this have the potentially serious problem of making errors/reads a function of a correlate of reads. We directly a Poisson error function, handling both this problem and heteroscedasticity. Binned data are to show linear shape only.pic.twitter.com/8vGe7ClsQ3
Protein abundance should affect selection, not sample preparation errors, so this is evidence that the higher error rate in E. coli than S. cerevisiae is real. @etienne_rajon and I predicted this countintuitive result in 2011 from drift barrier theory.https://www.pnas.org/content/108/3/1082 …
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