Cluster of 10 whole-cell recordings in the same slice live.
pic.twitter.com/rzU8ubPBDE
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Prikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi
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Hi Franz, this looks great! Is the prep for keeping human slices alive much different from rodent preps? We are interested in doing this too but have only patched rodent neurons.
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Hey Eric, you can check our recent elife paper (Peng et al. 2019). There we describe solutions & basic steps of the prep. But over all it’s pretty similar to slicing rodent tissue. Due to complex 3D structure of human necortex it’s a little harder to hit the right slicing angle.
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Beautiful recordings! Congrats!
Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi
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This is gorgeous.
Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi
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Awesome! (And I hope the surgery was a success for the person!).
Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi
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Just when I think I work in a tiny world (dentistry) you put me to shame :)
Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi
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Hi... that looks nice! Random question: what brand of camera and software are you using for patching?
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Thank you David! Check out our elife paper (Peng et al. 2019). There we describe the set up in detail. We use cellSens Dimension software and a Hamamatsu Orca Flash camera.
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| med-student | Geiger lab in Berlin