ExCyte

@ExCyted

ExCyte is a professional flow cytometry consulting company. Our mission is to provide expert instruction and to help leaders in the field connect and grow.

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Vrijeme pridruživanja: srpanj 2012.

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  1. prije 3 sata

    🔥 OPENING SOON: EXPERT CYTOMETRY WAITLIST Stop wasting time and money doing flow cytometry experiments incorrectly. Become an expert. Get published. Join the waitlist for first access to the expert training:

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  2. prije 5 sati

    We're about to go LIVE! [FREE WEBINAR] Advanced 4-18 Color Compensation Strategies For 2020 starts SOON at 1pm EST! Join us here:

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  3. prije 9 sati

    🧑‍🔬👩‍🔬 [WEBINAR TODAY] Advanced 4-18 Color Compensation Strategies For 2020 — join us at 1pm ET! *Reserve your seat:

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  4. 4. velj

    🧠 Ready to learn from the experts? Enrollment into the Expert Cytometry Master Class is opening soon! Join the waitlist for first access:

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  5. 4. velj

    Differential pressure establishes the size of the core stream. As the core stream increases in size, the change of coincident events increases, as does the spread of the data. It is recommended to run all the samples at the same flow rate, and use as low a flow rate as possible.

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  6. 3. velj

    🛑Stop wasting time and money doing flow cytometry experiments incorrectly. Become an expert. Get published. WAITLIST OPEN: Join now for first access to more information about the program and its benefits!🧑‍🔬

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  7. 3. velj

    Prepare for the SCYM exam by learning about some properties of light, and how filters and lenses are used to move the light around the optical bench of the flow cytometer.

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  8. 31. sij

    Teaching point: Learn about measuring and counting bacteria using flow cytometry as well as discover a new technique for sorting bacteria based on 16S rRNA sequence.

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  9. 30. sij

    Getting properly trained in flow cytometry, and keeping up-to-date with the flow cytometry best practices is required. This is our mission at Expert Cytometry: to ensure that your flow cytometry experiments are done properly and are reproducible.

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  10. 29. sij

    💡Voltage optimization may help improve separation. Not all channels will be improved, and it is important to make sure that the signal remain in the linear range of the PMT and on scale.

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  11. 27. sij

    🟢Using FMO and unstimulated controls to set gates (Maecker and Trotter, 2006). The FMO control addresses issues related to flurochrome spread, while the unstimulated helps address NSB binding. Combining the two ensures the gate is properly set.

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  12. 24. sij

    👀Teaching point: A review of how to considerations for generating single-cell preparations for flow cytometry.

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  13. 23. sij

    Fluid stability can impact signal detection. If there's a microclog on the waste side of the flow cell, it can cause pressure to build up, slowing the stream down and preventing the cells from hitting the proper window set by time delay.

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  14. 22. sij

    Differences between Std. Normal and T. Distribution.

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  15. 21. sij

    SSM for comparing instruments: This can help in quality control, as well as determining which instrument would be best for a given panel.

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  16. 20. sij

    👀 Don't over-interpret Spectral Viewers. Check the excitation max level...

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  17. 18. sij

    🤔 Sort windows - one or two drops? It will depend on the application and where the cell is within the drop.

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  18. 17. sij

    💡Learning tSNE using smaller panels. In this case a 5 color panel was used. By gating on regions of the panel, and displaying them using traditional plots, one gains confidence in the automated analysis.

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  19. 16. sij

    Signal Processing -- from photon to electron. The photocurrent from the PMT is sampled at a given rate, and the signal there is digitized based on the number of bins the ADC can put data into. Eventually it is stored as a listmode file.

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  20. 16. sij

    🟡 Learning tSNE using smaller panels -- In this case, a 5-color panel was used. By gating on regions of the panel, and displaying them using traditional plots, one gains confidence in the automated analysis.

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