Props to @TimWannier, @geochurch, and my new postdoc lab at @harvardmed @wyssinstitute for this remarkable project and the excellent collaboration!
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Of course, the corresponding pORTMAGE plasmids (in
@SEVAplasmids format) will be online shortly on@Addgene - to make these tools immediately accessible for everyone! https://www.biorxiv.org/content/10.1101/2020.01.14.906594v1 …#biotech#bio#SyntheticBiology#GenomeEngineering#pORTMAGE#Pseudomonaspic.twitter.com/fC0CcWb19A – mjesto: Harvard Medical School New Research Building
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Deposited plasmids: pORTMAGE-Ec1 https://www.addgene.org/138474/ pORTMAGE-Pa1 https://www.addgene.org/138475/ https://www.biorxiv.org/content/10.1101/2020.01.14.906594v1 …
@Addgene@SEVAplasmidspic.twitter.com/NX0SlHBS7R
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Very cool Akos! Congrats
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Thanks so much, Vivek! I hope this work and the plasmids will make it much easier for everyone to engineer bacterial/viral genomes, and plasmids in vivo.
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Every couple years I try to catch up on ssDNA binding protein literature (currently it's been a few) and my impression was always that no one really knows how they work for recombineering. Is that finally changing? Nice work btw...
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Hopefully, the field is getting closer to understand what's going on! I also highly recommend the recent paper of
@TimWannier@geochurch and cohttps://academic.oup.com/nar/article/47/4/1950/5280937 …
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Congratulations for great work
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Thanks Ranti!!
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Is that the one I saw you writing on the plane when we both went to GAPSummit and happened to sit next to each other? Looks familiar
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Thanks Melanie! It was this one, yes. Took some time and a few more transatlantic flights to wrap this story up. I hope you are doing great too!
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