Awesome article. “DNBSEQ-TX”, probably DNB=DNA NanoBall. p.s., if could adapt ~this for FISSEQ-ExM, could perhaps do in-situ barcode connectomics at speed... :-)
https://www.ncbi.nlm.nih.gov/m/pubmed/19892942/ …
https://arxiv.org/abs/1404.5103
https://www.biorxiv.org/content/10.1101/294637v1 … #dnananoballs #rolonies #brain #fasterhttps://twitter.com/antonioregalado/status/1232771616825892865 …
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Replying to @AdamMarblestone
nanoballs plus the readout is done with antibodies that affix to particular bases, rather than labelling the bases. I think they call that CoolMPS or something.
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Replying to @antonioregalado
Lots of dyes per antibody maybe, versus one per a directly labeled nucleotide. Hence more photons—>faster with cheaper, noisier cameras and faster, noisier streaming to disk... AWESOME
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Replying to @AdamMarblestone @antonioregalado
Oh my god this is too coolhttps://www.biorxiv.org/content/10.1101/2020.02.19.953307v1 …
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