nanoballs plus the readout is done with antibodies that affix to particular bases, rather than labelling the bases. I think they call that CoolMPS or something.
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Lots of dyes per antibody maybe, versus one per a directly labeled nucleotide. Hence more photons—>faster with cheaper, noisier cameras and faster, noisier streaming to disk... AWESOME
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Would be brighter, but diffusion might be an issue since antibodies are larger than fluorescent nucleotides.
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Yeah it is true, potentially a problem for FISSEQ. But maybe not, insomuch as you can do some post-expansion antibody staining, e.g., per recent Dawen Cai paper. The robotic dipping of big chips into big vats is pretty cool regardless though...
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