At 1 mm^3 / day, the human connectome would take ~3,500 years to image. The fastest EM approach (GCIB-SEM) is currently at 1 mm^3 / month. I suggest that we need an entirely new imaging paradigm to overcome this challenge! This is the challenge that my research focuses on.
Getting ~1000 GCIBs and microscopes is not that bad. I see reasons for entirely new imaging approaches, but not primarily on the basis of speed/time.
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If not speed-related, could you elaborate on what reasons you see for entirely new imaging approaches?
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Molecular content, foremost & robust long-distance reconstruction via barcoding. Perhaps collecting somewhat fewer voxels given barcoding, which would indeed improve speed. Simpler instrumentation, e.g., standard optical microscopes.
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