Opinion: electron microscopy resolution is unnecessary for connectomics, super-resolution light microscopy (or x-ray microscopy) will be sufficient if IHC tags for synapses are used, especially since these sorts of computational techniques are emerging:https://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1005493 …
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Replying to @LoganTCollins
Correct, if you have enough multiplexing to link the tag combinations back to their parent cell bodies:https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0008853 …
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Replying to @LoganTCollins
No problem. This is basically why I first got convinced that expansion microscopy plus in-situ multiplexing is ultimately going to be a good solution.
5:15 PM - 28 Oct 2019
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