Opinion: electron microscopy resolution is unnecessary for connectomics, super-resolution light microscopy (or x-ray microscopy) will be sufficient if IHC tags for synapses are used, especially since these sorts of computational techniques are emerging:https://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1005493 …
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Ooh, this looks amazing! Thanks for sharing
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No problem. This is basically why I first got convinced that expansion microscopy plus in-situ multiplexing is ultimately going to be a good solution.
End of conversation
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