Change my mind: EM will likely be the only viable tool for doing connectomics in excised human brain tissue, because optical tracing methods will require viral transfection in vitro that takes days/weeks, and tissue quality degrades quickly.
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I think I've seen images of neurites labeled with this technique in some of your previous talks. Always seemed promising, but I haven't seen any experimental follow-up. What were the sticking points?
Thanks. Twitter will use this to make your timeline better. UndoUndo
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